The present study investigate that evaluate the wound healing activity of the plant, ethanolic extract was prepared. A study was conducted to wound healing activity of Datura metel Linn. on albino rats of Wistar strain using three different models viz, excision incision and dead space wound was studied. The granulation tissue formed on days 4, 8, 12 and 16 (post-wound) was used to estimate total collagen, hexosamine, protein, DNA and uronic acid. The extract increased cellular proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and total collagen content of granulation tissues. The extract treated wounds were found to heal much faster as indicated by improved rates of epithelialization and wound contraction, also confirmed by histopathological examinations. The extract of Datura metel Linn., significantly increased the wound breaking strength in the incision wound model compared with controls (P < 0.001). The extract-treated wounds were found to epithelialize faster, and the rate of wound contraction was significantly increased in comparison to control wounds (P < 0.001), Wet and dry granulation tissue weights in a dead space wound model increased significantly (p < 0.001). There was a significant increase in wound closure rate, tensile strength, dry granuloma weight, wet granuloma weight and decrease in epithelization period in Datura metel Linn, treated group when compared to control and commercial drug treated groups. From the results, it may be concluded that the ethanolic extract of Datura metel Linn, had greater wound healing activity than the nitrofurazone ointment
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